TY - JOUR T1 - Small ribonucleoproteins from eukaryotes: structures and roles in RNA biogenesis. JF - Cold Spring Harb Symp Quant Biol Y1 - 1983 A1 - Steitz, J A A1 - Wolin, S L A1 - Rinke, J A1 - Pettersson, I A1 - Mount, S M A1 - Lerner, E A A1 - Hinterberger, M A1 - Gottlieb, E KW - Animals KW - Base Sequence KW - HeLa Cells KW - HUMANS KW - Mice KW - Molecular Weight KW - Nucleic Acid Conformation KW - Nucleic Acid Hybridization KW - Nucleoproteins KW - Ribonucleoproteins KW - Ribonucleoproteins, Small Nuclear KW - RNA Polymerase III KW - Transcription, Genetic VL - 47 Pt 2 ER - TY - JOUR T1 - The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro. JF - Cell Y1 - 1983 A1 - Mount, S M A1 - Pettersson, I A1 - Hinterberger, M A1 - Karmas, A A1 - Steitz, J A KW - Base Sequence KW - DNA-Directed RNA Polymerases KW - HUMANS KW - Nucleoproteins KW - Ribonuclease T1 KW - Ribonucleoproteins KW - Ribonucleoproteins, Small Nuclear KW - RNA KW - RNA Splicing KW - T-Phages AB -

The ability of purified U1 small nuclear RNA-protein complexes (U1 snRNPs) to bind in vitro to two RNAs transcribed from recombinant DNA clones by bacteriophage T7 RNA polymerase has been studied. A transcript which contains sequences corresponding to the small intron and flanking exons of the major mouse beta-globin gene is bound in marked preference to an RNA devoid of splice site sequences. The site of U1 snRNP binding to the globin RNA has been defined by T1 ribonuclease digestion of the RNA-U1 snRNP complex. A 15-17-nucleotide region, including the 5' splice site, remains undigested and complexed with the snRNP such that it can be co-precipitated by antibodies directed against the U1 snRNP. Partial proteinase K digestion of the U1 snRNP abolishes interaction with the globin RNA, indicating that the snRNP proteins contribute significantly to RNA binding. No RNA cleavage, splicing, or recognition of the 3' splice site by U1 snRNPs has been detected. Our results are discussed in terms of the probable role of U1 snRNPs in the messenger RNA splicing of eucaryotic cell nuclei.

VL - 33 CP - 2 ER - TY - JOUR T1 - Structure and function of small ribonucleoproteins from eukaryotic cells. JF - Princess Takamatsu Symp Y1 - 1982 A1 - Steitz, J A A1 - Berg, C A1 - Gottlieb, E A1 - Hardin, J A A1 - Hashimoto, C A1 - Hendrick, J P A1 - Hinterberger, M A1 - Krikeles, M A1 - Lerner, M R A1 - Mount, S M KW - Antigen-Antibody Complex KW - Autoantibodies KW - HUMANS KW - Lupus Erythematosus, Systemic KW - Nucleoproteins KW - Ribonucleoproteins KW - RNA Polymerase III KW - Transcription, Genetic AB -

Autoantibodies from patients with systemic lupus erythematosus and other related diseases have been used to identify and study small RNA-protein complexes from mammalian cells. Properties of three previously described and several new classes of small ribonucleoproteins (RNPs) are reviewed. The sequence of Drosophila U1 RNA reveals that the region proposed to pair with 5' splice junctions is conserved, while that proposed to interact with 3' junctions diverges; this forces some revision of the model for U1 small nuclear (sn)RNP participation in hnRNA splicing. Further characterization of the Ro and La small RNPs has shown that the Ro small cytoplasmic (sc)RNPs are a subclass of La RNPs. Both tRNA and 5S rRNA precursors are at least transiently associated with the La protein. This raises the possibility that the La protein may be an RNA polymerase III transcription factor.

VL - 12 ER - TY - JOUR T1 - Are snRNPs involved in splicing? JF - Nature Y1 - 1980 A1 - Lerner, M R A1 - Boyle, J A A1 - Mount, S M A1 - Wolin, S L A1 - Steitz, J A KW - Animals KW - Base Sequence KW - Cell Line KW - Chickens KW - Erythrocytes KW - HUMANS KW - Liver KW - Lupus Erythematosus, Systemic KW - Molecular Weight KW - Nucleic Acid Precursors KW - Nucleoproteins KW - Ribonucleoproteins KW - RNA, Heterogeneous Nuclear KW - Species Specificity VL - 283 CP - 5743 ER -