@article {38313,
	title = {Genomic analysis of ICEVchBan8: An atypical genetic element in Vibrio cholerae},
	journal = {FEBS LettersFEBS Letters},
	year = {2012},
	type = {10.1016/j.febslet.2012.03.064},
	abstract = {Genomic islands (GIs) and integrative conjugative elements (ICEs) are major players in bacterial evolution since they encode genes involved in adaptive functions of medical or environmental importance. Here we performed the genomic analysis of ICEVchBan8, an unusual ICE found in the genome of a clinical non-toxigenic Vibrio cholerae O37 isolate. ICEVchBan8 shares most of its genetic structure with SXT/R391 ICEs. However, this ICE codes for a different integration/excision module is located at a different insertion site, and part of its genetic cargo shows homology to other pathogenicity islands of V. cholerae.},
	keywords = {Genomic islands, Integrative conjugative elements, Lateral gene transfer, Vibrio cholerae},
	isbn = {0014-5793},
	author = {Taviani, Elisa and Spagnoletti, Matteo and Ceccarelli, Daniela and Haley, Bradd J. and Hasan, Nur A. and Chen, Arlene and Colombo, Mauro M. and Huq, Anwar and Rita R. Colwell}
}
@article {38207,
	title = {Discovery of novel Vibrio cholerae VSP-II genomic islands using comparative genomic analysis},
	journal = {FEMS Microbiology LettersFEMS Microbiology Letters},
	volume = {308},
	year = {2010},
	type = {10.1111/j.1574-6968.2010.02008.x},
	abstract = {This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presence of three novel VSP-II in clinical and environmental V. cholerae marked by major deletions and genetic rearrangements. A new VSP-II cluster was found in the seventh pandemic V. cholerae O1 El Tor strain CIRS101, which is dominant (95\%) among the recent (2004{\textendash}2007) seven pandemic V. cholerae O1 El Tor isolates from two endemic sites, but was not found in older strains from the same region. Two other variants were found in V. cholerae TMA21 and RC385, two environmental strains from coastal Brazil and the Chesapeake Bay, respectively, the latter being prevalent among environmental V. cholerae non-O1/non-O139 and Vibrio mimicus. The results of this study indicate that the VSP-II island has undergone significant rearrangement through a complex evolutionary pathway in V. cholerae. Interestingly, one of the new VSP-II revealed the presence of {\textquoteleft}old{\textquoteright} and {\textquoteleft}new{\textquoteright}V. cholerae O1 El Tor pandemic clones circulating in some of the areas where cholera is endemic.},
	keywords = {Vibrio cholerae, Vibrio mimicus, VPS-II},
	isbn = {1574-6968},
	author = {Taviani, Elisa and Grim, Christopher J. and Choi, Jinna and Jongsik, Chun and Haley, Bradd and Hasan, Nur A. and Huq, Anwar and Rita R. Colwell}
}
@article {38202,
	title = {Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns},
	journal = {Research in MicrobiologyResearch in Microbiology},
	volume = {160},
	year = {2009},
	type = {10.1016/j.resmic.2008.10.008},
	abstract = {Sequencing of three housekeeping genes, mdh, dnaE and recA, and ribotyping for seven non-toxigenic Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three toxigenic strains (N16961, O395, and 569B) and three non-toxigenic strains from India (GS1, GS2, and GW87) and one Guam strain (X392), the latter of which were similar in both MLST and ribotyping, while two other non-toxigenic strains from the USA and India (2740-80 and OR69) appeared to be more closely related to toxigenic strains than to non-toxigenic strains, although this was not supported by ribotyping. These results provide clues to the emergence of toxigenic strains from a non-toxigenic progenitor by acquisition of virulence gene clusters. Results of split decomposition analysis suggest that widespread recombination occurs among the three housekeeping genes and that recombination plays an important role in the emergence of toxigenic strains of V. cholerae O1.},
	keywords = {Housekeeping genes, Ribotyping, sequencing, Vibrio cholerae},
	isbn = {0923-2508},
	author = {Mohapatra, Saswat S. and Ramachandran, Dhanya and Mantri, Chinmay K. and Rita R. Colwell and Singh, Durg V.}
}
@inbook {38199,
	title = {Detection, Isolation, and Identification of Vibrio cholerae from the Environment},
	booktitle = {Current Protocols in MicrobiologyCurrent Protocols in Microbiology},
	year = {2006},
	publisher = {John Wiley \& Sons, Inc.},
	organization = {John Wiley \& Sons, Inc.},
	keywords = {culturable, DETECTION, Environment, identification, isolation, nonculturable, viable, Vibrio cholerae},
	isbn = {9780471729259},
	author = {Huq, Anwar and Grim, Christopher and Rita R. Colwell and Nair, G. Balakrish}
}
@article {38488,
	title = {Septaplex PCR assay for rapid identification of Vibrio cholerae including detection of virulence and int SXT genes},
	journal = {FEMS Microbiology LettersFEMS Microbiology Letters},
	volume = {265},
	year = {2006},
	type = {10.1111/j.1574-6968.2006.00491.x},
	abstract = {In this study, we describe a septaplex PCR assay for rapid identification of Vibrio cholerae including detection of the virulence and intsxt genes. Conditions were optimized to amplify fragments of ISRrRNA (encoding for 16S{\textendash}23S rRNA gene, Intergenic spacer regions), O1rfb (O1 serogroup specific rfb), O139rfb (O139 serogroup specific rfb), ctxA (cholera toxin subunit A), tcpA (toxin coregulated pilus), and intsxt (sxt integron) simultaneously in a single PCR. The septaplex PCR was evaluated using 211 strains of V. cholerae and six water samples for in situ testing. PCR results were correlated with genotype data obtained by individual PCR and slot-blot assays. The one-step PCR described here can be used to identify V. cholerae accurately and rapidly. Also, the virulence and intsxt genes can be simultaneously detected, providing a useful method for monitoring pathogenic, intsxt-positive and nonpathogenic, intsxt-negative V. cholerae serogroups both in the environment and clinical settings.},
	keywords = {DETECTION, intsxt, septaplex PCR, Vibrio cholerae, virulence},
	isbn = {1574-6968},
	author = {Mantri, Chinmay K. and Mohapatra, Saswat S. and Ramamurthy, Thandavarayan and Ghosh, Raikamal and Rita R. Colwell and Singh, Durg V.}
}
