TY - JOUR T1 - Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia JF - Applied and Environmental MicrobiologyAppl. Environ. Microbiol.Applied and Environmental MicrobiologyAppl. Environ. Microbiol. Y1 - 2010 A1 - Whitehouse, Chris A. A1 - Baldwin, Carson A1 - Sampath, Rangarajan A1 - Blyn, Lawrence B. A1 - Melton, Rachael A1 - Li, Feng A1 - Hall, Thomas A. A1 - Harpin, Vanessa A1 - Matthews, Heather A1 - Tediashvili, Marina A1 - Jaiani, Ekaterina A1 - Kokashvili, Tamar A1 - Janelidze, Nino A1 - Grim, Christopher A1 - Rita R. Colwell A1 - Huq, Anwar AB - The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected. VL - 76 SN - 0099-2240, 1098-5336 ER -