TY - JOUR T1 - Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans. JF - Insect Mol Biol Y1 - 2006 A1 - Attardo, G M A1 - Strickler-Dinglasan, P A1 - Perkin, S A H A1 - Caler, E A1 - Bonaldo, M F A1 - Soares, M B A1 - El-Sayeed, N A1 - Aksoy, S KW - Adipose Tissue KW - Animals KW - Base Sequence KW - Computational Biology KW - DNA Primers KW - Egg Proteins KW - Expressed Sequence Tags KW - Female KW - Gene Expression Profiling KW - Insect Vectors KW - Male KW - Molecular Sequence Data KW - Reverse Transcriptase Polymerase Chain Reaction KW - Sequence Analysis, DNA KW - Sex Factors KW - Tsetse Flies AB -
Tsetse flies (Diptera: Glossinidia) are vectors of pathogenic African trypanosomes. To develop a foundation for tsetse physiology, a normalized expressed sequence tag (EST) library was constructed from fat body tissue of immune-stimulated Glossina morsitans morsitans. Analysis of 20,257 high-quality ESTs yielded 6372 unique genes comprised of 3059 tentative consensus (TC) sequences and 3313 singletons (available at http://aksoylab.yale.edu). We analysed the putative fat body transcriptome based on homology to other gene products with known functions available in the public domain. In particular, we describe the immune-related products, reproductive function related yolk proteins and milk-gland protein, iron metabolism regulating ferritins and transferrin, and tsetse's major energy source proline biosynthesis. Expression analysis of the three yolk proteins indicates that all are detected in females, while only the yolk protein with similarity to lipases, is expressed in males. Milk gland protein, apparently important for larval nutrition, however, is primarily synthesized by accessory milk gland tissue.
VL - 15 CP - 4 M3 - 10.1111/j.1365-2583.2006.00649.x ER -