TY - JOUR T1 - Characterization of Pseudoalteromonas citrea and P. nigrifaciens Isolated from Different Ecological Habitats Based on REP-PCR Genomic Fingerprints JF - Systematic and Applied MicrobiologySystematic and Applied Microbiology Y1 - 2002 A1 - Ivanova, Elena P. A1 - Matte, Glavur R. A1 - Matte, Maria H. A1 - Coenye, Tom A1 - Huq, Anwarul A1 - Rita R. Colwell KW - biogeography KW - BOX-PCR KW - ERIC KW - Pseudoalteromonas KW - REP AB - SummaryDNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, Gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67–70% DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion. VL - 25 SN - 0723-2020 ER -