TY - JOUR T1 - Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus. JF - Nat Biotechnol Y1 - 2007 A1 - Myers, Garry S A A1 - Parker, Dane A1 - Al-Hasani, Keith A1 - Kennan, Ruth M A1 - Seemann, Torsten A1 - Ren, Qinghu A1 - Badger, Jonathan H A1 - Selengut, Jeremy D A1 - DeBoy, Robert T A1 - Tettelin, Hervé A1 - Boyce, John D A1 - McCarl, Victoria P A1 - Han, Xiaoyan A1 - Nelson, William C A1 - Madupu, Ramana A1 - Mohamoud, Yasmin A1 - Holley, Tara A1 - Fedorova, Nadia A1 - Khouri, Hoda A1 - Bottomley, Steven P A1 - Whittington, Richard J A1 - Adler, Ben A1 - Songer, J Glenn A1 - Rood, Julian I A1 - Paulsen, Ian T KW - Animals KW - Antigens KW - Chromosome mapping KW - Dichelobacter nodosus KW - Foot Rot KW - Genome, Bacterial KW - Sequence Analysis, DNA AB -

Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

VL - 25 CP - 5 M3 - 10.1038/nbt1302 ER - TY - JOUR T1 - Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus JF - Nature biotechnologyNature biotechnology Y1 - 2007 A1 - Myers, Garry S. A. A1 - Parker, Dane A1 - Al-Hasani, Keith A1 - Kennan, Ruth M. A1 - Seemann, Torsten A1 - Ren, Qinghu A1 - Badger, Jonathan H. A1 - J. Selengut A1 - DeBoy, Robert T. A1 - Tettelin, Hervé A1 - Boyce, John D. A1 - McCarl, Victoria P. A1 - Han, Xiaoyan A1 - Nelson, William C. A1 - Madupu, Ramana A1 - Mohamoud, Yasmin A1 - Holley, Tara A1 - Fedorova, Nadia A1 - Khouri, Hoda A1 - Bottomley, Steven P. A1 - Whittington, Richard J. A1 - Adler, Ben A1 - Songer, J. Glenn A1 - Rood, Julian I. A1 - Paulsen, Ian T. KW - Animals KW - Antigens KW - Chromosome mapping KW - Dichelobacter nodosus KW - Foot Rot KW - Genome, Bacterial KW - Sequence Analysis, DNA AB - Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine. VL - 25 N1 - http://www.ncbi.nlm.nih.gov/pubmed/17468768?dopt=Abstract ER - TY - JOUR T1 - Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. JF - Cell Y1 - 1983 A1 - Padgett, R A A1 - Mount, S M A1 - Steitz, J A A1 - Sharp, P A KW - Adenoviruses, Human KW - Antigens KW - Autoantigens KW - Base Sequence KW - Cell Extracts KW - HeLa Cells KW - HUMANS KW - Immune Sera KW - Nucleic Acid Precursors KW - Ribonucleoproteins KW - Ribonucleoproteins, Small Nuclear KW - RNA KW - RNA Precursors KW - RNA Splicing KW - RNA, Messenger KW - RNA, Small Cytoplasmic KW - RNA, Viral KW - Transcription, Genetic AB -

A mouse monoclonal antibody and human autoimmune sera directed against various classes of small ribonucleoprotein particles have been tested for inhibition of mRNA splicing in a soluble in vitro system. The splicing of the first and second leader exons of adenovirus late RNA was inhibited only by those sera that reacted with U1 RNP. Both U1 RNP-specific human autoimmune serum and sera directed against the Sm class of small nuclear RNPs, including a mouse monoclonal antibody, specifically inhibited splicing. Antisera specific for U2 RNP had no effect on splicing nor did antisera specific for the La or Ro class of small RNPs. These results suggest that U1 RNP is essential for the splicing of mRNA precursors.

VL - 35 CP - 1 ER -