TY - JOUR T1 - Small ribonucleoproteins from eukaryotes: structures and roles in RNA biogenesis. JF - Cold Spring Harb Symp Quant Biol Y1 - 1983 A1 - Steitz, J A A1 - Wolin, S L A1 - Rinke, J A1 - Pettersson, I A1 - Mount, S M A1 - Lerner, E A A1 - Hinterberger, M A1 - Gottlieb, E KW - Animals KW - Base Sequence KW - HeLa Cells KW - HUMANS KW - Mice KW - Molecular Weight KW - Nucleic Acid Conformation KW - Nucleic Acid Hybridization KW - Nucleoproteins KW - Ribonucleoproteins KW - Ribonucleoproteins, Small Nuclear KW - RNA Polymerase III KW - Transcription, Genetic VL - 47 Pt 2 ER - TY - JOUR T1 - Structure and function of small ribonucleoproteins from eukaryotic cells. JF - Princess Takamatsu Symp Y1 - 1982 A1 - Steitz, J A A1 - Berg, C A1 - Gottlieb, E A1 - Hardin, J A A1 - Hashimoto, C A1 - Hendrick, J P A1 - Hinterberger, M A1 - Krikeles, M A1 - Lerner, M R A1 - Mount, S M KW - Antigen-Antibody Complex KW - Autoantibodies KW - HUMANS KW - Lupus Erythematosus, Systemic KW - Nucleoproteins KW - Ribonucleoproteins KW - RNA Polymerase III KW - Transcription, Genetic AB -

Autoantibodies from patients with systemic lupus erythematosus and other related diseases have been used to identify and study small RNA-protein complexes from mammalian cells. Properties of three previously described and several new classes of small ribonucleoproteins (RNPs) are reviewed. The sequence of Drosophila U1 RNA reveals that the region proposed to pair with 5' splice junctions is conserved, while that proposed to interact with 3' junctions diverges; this forces some revision of the model for U1 small nuclear (sn)RNP participation in hnRNA splicing. Further characterization of the Ro and La small RNPs has shown that the Ro small cytoplasmic (sc)RNPs are a subclass of La RNPs. Both tRNA and 5S rRNA precursors are at least transiently associated with the La protein. This raises the possibility that the La protein may be an RNA polymerase III transcription factor.

VL - 12 ER - TY - JOUR T1 - Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract. JF - Nucleic Acids Res Y1 - 1981 A1 - Dingermann, T A1 - Sharp, S A1 - Appel, B A1 - DeFranco, D A1 - Mount, S A1 - Heiermann, R A1 - Pongs, O A1 - Söll, D KW - Animals KW - Cell-Free System KW - Cloning, Molecular KW - Drosophila KW - In Vitro Techniques KW - RNA KW - RNA Polymerase III KW - RNA, Transfer KW - Transcription, Genetic KW - Xenopus laevis AB -

We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.

VL - 9 CP - 16 ER -