Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus.

TitleGenome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus.
Publication TypeJournal Articles
Year of Publication2007
AuthorsMyers GSA, Parker D, Al-Hasani K, Kennan RM, Seemann T, Ren Q, Badger JH, Selengut JD, DeBoy RT, Tettelin H, Boyce JD, McCarl VP, Han X, Nelson WC, Madupu R, Mohamoud Y, Holley T, Fedorova N, Khouri H, Bottomley SP, Whittington RJ, Adler B, J Songer G, Rood JI, Paulsen IT
JournalNat Biotechnol
Volume25
Issue5
Pagination569-75
Date Published2007 May
ISSN1087-0156
KeywordsAnimals, Antigens, Chromosome mapping, Dichelobacter nodosus, Foot Rot, Genome, Bacterial, Sequence Analysis, DNA
Abstract

Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

DOI10.1038/nbt1302
Alternate JournalNat. Biotechnol.
PubMed ID17468768