P element-mediated in vivo deletion analysis of white-apricot: deletions between direct repeats are strongly favored.

TitleP element-mediated in vivo deletion analysis of white-apricot: deletions between direct repeats are strongly favored.
Publication TypeJournal Articles
Year of Publication1994
AuthorsKurkulos M, Weinberg JM, Roy D, Mount SM
JournalGenetics
Volume136
Issue3
Pagination1001-11
Date Published1994 Mar
ISSN0016-6731
KeywordsAlleles, Animals, Animals, Genetically Modified, Base Sequence, Crosses, Genetic, DNA, DNA Transposable Elements, Drosophila, Eye Color, Female, Genes, Insect, Male, Molecular Sequence Data, Nucleotidyltransferases, PHENOTYPE, Recombination, Genetic, Repetitive Sequences, Nucleic Acid, Sequence Deletion, Transformation, Genetic, Transposases
Abstract

We have isolated and characterized deletions arising within a P transposon, P[hswa], in the presence of P transposase. P[hswa] carries white-apricot (wa) sequences, including a complete copia element, under the control of an hsp70 promoter, and resembles the original wa allele in eye color phenotype. In the presence of P transposase, P[hswa] shows a high overall rate (approximately 3%) of germline mutations that result in increased eye pigmentation. Of 234 derivatives of P[hswa] with greatly increased eye pigmentation, at least 205 carried deletions within copia. Of these, 201 were precise deletions between the directly repeated 276-nucleotide copia long terminal repeats (LTRs), and four were unique deletions. High rates of transposase-induced precise deletion were observed within another P transposon carrying unrelated 599 nucleotide repeats (yeast 2 mu FLP; recombinase target sites) separated by 5.7 kb. Our observation that P element-mediated deletion formation occurs preferentially between direct repeats suggests general methods for controlling deletion formation.

Alternate JournalGenetics
PubMed ID8005410
PubMed Central IDPMC1205858
Grant ListGM37991 / GM / NIGMS NIH HHS / United States