Polyadenylylation in copia requires unusually distant upstream sequences.

TitlePolyadenylylation in copia requires unusually distant upstream sequences.
Publication TypeJournal Articles
Year of Publication1991
AuthorsKurkulos M, Weinberg JM, Pepling ME, Mount SM
JournalProc Natl Acad Sci U S A
Date Published1991 Apr 15
KeywordsAnimals, Base Sequence, Blotting, Northern, DNA Transposable Elements, Drosophila melanogaster, Eye Color, Molecular Sequence Data, Oligonucleotides, Polymerase Chain Reaction, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, RNA Processing, Post-Transcriptional, RNA, Messenger

Retroviruses and related genetic elements generate terminally redundant RNA products by differential polyadenylylation within a long terminal repeat. Expression of the white-apricot (wa) allele of Drosophila melanogaster, which carries an insertion of the 5.1-kilobase retrovirus-like transposable element copia in a small intron, is influenced by signals within copia. By using this indicator, we have isolated a 518-base-pair deletion, 312 base pairs upstream of the copia polyadenylylation site, that is phenotypically like much larger deletions and eliminates RNA species polyadenylylated in copia. This requirement of distant upstream sequences for copia polyadenylylation has implications for the expression of many genetic elements bearing long terminal repeats.

Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID1849643
PubMed Central IDPMC51379
Grant ListGM 37991 / GM / NIGMS NIH HHS / United States