Sequencing strategies for parasite genomes.

TitleSequencing strategies for parasite genomes.
Publication TypeJournal Articles
Year of Publication2004
AuthorsBartholomeu D, El-Sayed NM
JournalMethods Mol Biol
Date Published2004
KeywordsAnimals, Chromosome Walking, Chromosomes, Artificial, Bacterial, Genetic Markers, Genome, Protozoan, Plasmodium falciparum

Recent advances in the field of sequencing have enabled the determination of the complete nucleotide sequence of a large number of complex genomes. The complete genome sequence of the parasite Plasmodium falciparum has been published recently, and many other parasite genome initiatives are underway. Parasite genomes vary in size, nucleotide composition, polymorphism level, content, and distribution of repetitive elements. These genomic features affect the performance of sequencing strategies. As a consequence, each of the ongoing parasite genome projects has adopted distinct sequencing approaches. The degree of completeness and accuracy desired as well as available funds should be considered carefully when choosing the most appropriate sequencing strategy.

Alternate JournalMethods Mol. Biol.
PubMed ID15153620