Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract.
| Title | Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract. |
| Publication Type | Journal Articles |
| Year of Publication | 1981 |
| Authors | Dingermann T, Sharp S, Appel B, DeFranco D, Mount S, Heiermann R, Pongs O, Söll D |
| Journal | Nucleic Acids Res |
| Volume | 9 |
| Issue | 16 |
| Pagination | 3907-18 |
| Date Published | 1981 Aug 25 |
| ISSN | 0305-1048 |
| Keywords | Animals, Cell-Free System, Cloning, Molecular, Drosophila, In Vitro Techniques, RNA, RNA Polymerase III, RNA, Transfer, Transcription, Genetic, Xenopus laevis |
| Abstract | We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. |
| Alternate Journal | Nucleic Acids Res. |
| PubMed ID | 6170932 |
| PubMed Central ID | PMC327404 |