Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia
| Title | Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia |
| Publication Type | Journal Articles |
| Year of Publication | 2010 |
| Authors | Whitehouse CA, Baldwin C, Sampath R, Blyn LB, Melton R, Li F, Hall TA, Harpin V, Matthews H, Tediashvili M, Jaiani E, Kokashvili T, Janelidze N, Grim C, Colwell RR, Huq A |
| Journal | Applied and Environmental MicrobiologyAppl. Environ. Microbiol.Applied and Environmental MicrobiologyAppl. Environ. Microbiol. |
| Volume | 76 |
| Type of Article | 10.1128/AEM.01919-09 |
| ISBN Number | 0099-2240, 1098-5336 |
| Abstract | The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected. |