Genome assembly forensics: finding the elusive mis-assembly

TitleGenome assembly forensics: finding the elusive mis-assembly
Publication TypeJournal Articles
Year of Publication2008
AuthorsPhillippy AM, Schatz MC, Pop M.
JournalGenome BiologyGenome Biology
Type of Article10.1186/gb-2008-9-3-r55
ISBN Number1465-6906

We present the first collection of tools aimed at automated genome assembly validation. This work formalizes several mechanisms for detecting mis-assemblies, and describes their implementation in our automated validation pipeline, called amosvalidate. We demonstrate the application of our pipeline in both bacterial and eukaryotic genome assemblies, and highlight several assembly errors in both draft and finished genomes. The software described is compatible with common assembly formats and is released, open-source, at