Measuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays.

TitleMeasuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays.
Publication TypeJournal Articles
Year of Publication2009
AuthorsBloom JS, Khan Z, Kruglyak L, Singh M, Caudy AA
JournalBMC Genomics
Volume10
Pagination221
Date Published2009
ISSN1471-2164
Keywordsalgorithms, DNA, Complementary, DNA, Fungal, Gene Expression Profiling, Oligonucleotide Array Sequence Analysis, Saccharomyces cerevisiae, sequence alignment, Sequence Analysis, DNA
Abstract

BACKGROUND: High-throughput cDNA synthesis and sequencing of poly(A)-enriched RNA is rapidly emerging as a technology competing to replace microarrays as a quantitative platform for measuring gene expression.

RESULTS: Consequently, we compared full length cDNA sequencing to 2-channel gene expression microarrays in the context of measuring differential gene expression. Because of its comparable cost to a gene expression microarray, our study focused on the data obtainable from a single lane of an Illumina 1 G sequencer. We compared sequencing data to a highly replicated microarray experiment profiling two divergent strains of S. cerevisiae.

CONCLUSION: Using a large number of quantitative PCR (qPCR) assays, more than previous studies, we found that neither technology is decisively better at measuring differential gene expression. Further, we report sequencing results from a diploid hybrid of two strains of S. cerevisiae that indicate full length cDNA sequencing can discover heterozygosity and measure quantitative allele-specific expression simultaneously.

DOI10.1186/1471-2164-10-221
Alternate JournalBMC Genomics
PubMed ID19435513
PubMed Central IDPMC2686739
Grant ListP50GM071508 / GM / NIGMS NIH HHS / United States
R37 MH059520 / MH / NIMH NIH HHS / United States