Quantitative measurement of allele-specific protein expression in a diploid yeast hybrid by LC-MS.
Title | Quantitative measurement of allele-specific protein expression in a diploid yeast hybrid by LC-MS. |
Publication Type | Journal Article |
Authors | Khan Z, Bloom JS, Amini S, Singh M, Perlman DH, Caudy AA, Kruglyak L |
2012 | |
Journal | Mol Syst Biol |
Volume | 8 |
Pages | 602 |
Alleles, Chromatography, Liquid, Fungal Proteins, Gene Expression Profiling, Gene Expression Regulation, Fungal, HUMANS, Mass Spectrometry, proteomics, Regression Analysis, Saccharomyces, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Species Specificity | |
Understanding the genetic basis of gene regulatory variation is a key goal of evolutionary and medical genetics. Regulatory variation can act in an allele-specific manner (cis-acting) or it can affect both alleles of a gene (trans-acting). Differential allele-specific expression (ASE), in which the expression of one allele differs from another in a diploid, implies the presence of cis-acting regulatory variation. While microarrays and high-throughput sequencing have enabled genome-wide measurements of transcriptional ASE, methods for measurement of protein ASE (pASE) have lagged far behind. We describe a flexible, accurate, and scalable strategy for measurement of pASE by liquid chromatography-coupled mass spectrometry (LC-MS). We apply this approach to a hybrid between the yeast species Saccharomyces cerevisiae and Saccharomyces bayanus. Our results provide the first analysis of the relative contribution of cis-acting and trans-acting regulatory differences to protein expression divergence between yeast species. | |
10.1038/msb.2012.34 | |
PubMed ID | 22893000 |
PubMed Central ID | PMC3435501 |
Grant List | P50 GM071508 / GM / NIGMS NIH HHS / United States R37 MH59520 / MH / NIMH NIH HHS / United States / / Howard Hughes Medical Institute / United States |