cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite

TitlecDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite
Publication TypeJournal Articles
Year of Publication1995
AuthorsEl‐Sayed NM, Alarcon CM, Beck JC, Sheffield VC, Donelson JE
JournalMolecular and Biochemical ParasitologyMolecular and Biochemical Parasitology
Volume73
Type of Article16/0166-6851(95)00098-L
ISBN Number0166-6851
KeywordscDNA, Expressed sequence tag, Trypanosoma brucei rhodesiense
Abstract

A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.