Simple Procedure for Rapid Identification of Vibrio Cholerae from the Aquatic Environment

TitleSimple Procedure for Rapid Identification of Vibrio Cholerae from the Aquatic Environment
Publication TypeJournal Articles
Year of Publication2002
AuthorsChoopun N, Louis V, Huq A, Colwell RR
JournalApplied and Environmental MicrobiologyAppl. Environ. Microbiol.Applied and Environmental MicrobiologyAppl. Environ. Microbiol.
Volume68
Type of Article10.1128/AEM.68.2.995-998.2002
ISBN Number0099-2240, 1098-5336
Abstract

Biochemical tests commonly used to screen for Vibrio cholerae in environmental samples were evaluated, and we found that a combination of alkaline peptone enrichment followed by streaking on thiosulfate citrate bile salts sucrose agar and testing for arginine dihydrolase activity and esculin hydrolysis was an effective rapid technique to screen for aquatic environmental V. cholerae. This technique provided 100% sensitivity and ≥70% specificity.